{ "engine": "bio-suite codon-opt-compare (Synonymous Codon CAI/GC3/Uridine Optimizer + Turner-2004 Zuker-McCaskill MFE)", "benchmark_id": "TOOL-BENCHMARK-20-CODON-OPT-VS-VIENNARNA", "validation_set_id": "VAL-SET-009-CODON-OPT-VIENNARNA-12COHORT", "external_reference": "ViennaRNA v2.7.2 (RNAfold -p --noPS: Turner-2004 MFE & McCaskill Partition Function) + Kazusa Homo sapiens (9606) Codon Usage Table", "total_cohorts": 12, "dev_cohorts": 6, "reserve_cohorts": 6, "mean_aa_identity_pct": 100.0, "mean_wt_cai": 0.684, "mean_opt_cai": 1.0, "mean_wt_uridine_pct": 34.2593, "mean_opt_uridine_pct": 17.4074, "dev_mean_mfe_abs_diff_kcal": 0.0, "reserve_mean_mfe_abs_diff_kcal": 0.0, "dev_mean_ens_abs_diff_kcal": 0.0, "reserve_mean_ens_abs_diff_kcal": 0.0, "overall_mfe_pearson_r": 1.0, "overall_ens_pearson_r": 1.0, "regime_concordance_pct": 100.0, "total_native_ms": 72.5662, "total_viennarna_ms": 760.7847, "speedup_factor": 10.484, "cohorts": [ { "cohort_id": "DEV-01", "split": "Development", "name": "SARS-CoV-2 Spike RBD Receptor-Binding Ridge (BNT162b2 / mRNA-1273 Target)", "organism_table": "Homo sapiens (9606)", "accession": "NC_045512.2:22910..22963", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MNNLNSKVGGNYNYLYRL", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.717, "optimized_cai": 1.0, "wildtype_gc3_pct": 33.3333, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 37.037, "optimized_uridine_pct": 14.8148, "wildtype_cpg_count": 0, "optimized_cpg_count": 1, "gen1_placeholder_mfe_error_kcal": 20.8689, "native_wt_mfe_kcal": -5.9, "viennarna_wt_mfe_kcal": -5.9, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -13.3, "viennarna_opt_mfe_kcal": -13.3, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -14.56, "viennarna_opt_ensemble_kcal": -14.56, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -7.4, "tir5_window_mfe_kcal": 0.0, "wildtype_mfe_structure": "...............((((.....((((((.......))))))....))))...", "optimized_mfe_structure": ".....((.((((.((((..((((((.......))))..))..)))).)))).))", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.717 -> 1.000, ΔMFE = -7.40 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 6.0923, "viennarna_runtime_ms": 343.9 }, { "cohort_id": "DEV-02", "split": "Development", "name": "Influenza A (H1N1) Hemagglutinin Stalk Fusion Peptide Epitope", "organism_table": "Homo sapiens (9606)", "accession": "NC_026433.1:1030..1083", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MGLFGAIAGFIENGWEGL", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.563, "optimized_cai": 1.0, "wildtype_gc3_pct": 16.6667, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 37.037, "optimized_uridine_pct": 18.5185, "wildtype_cpg_count": 0, "optimized_cpg_count": 6, "gen1_placeholder_mfe_error_kcal": 14.0689, "native_wt_mfe_kcal": -3.8, "viennarna_wt_mfe_kcal": -3.8, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -20.1, "viennarna_opt_mfe_kcal": -20.1, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -21.54, "viennarna_opt_ensemble_kcal": -21.54, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -16.3, "tir5_window_mfe_kcal": -5.4, "wildtype_mfe_structure": "....((((((((((.((((....)))).))).....)))))))...........", "optimized_mfe_structure": "..((((((.(((((((((......))).....((....)))))))).)))))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.563 -> 1.000, ΔMFE = -16.30 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 8.0186, "viennarna_runtime_ms": 37.8527 }, { "cohort_id": "DEV-03", "split": "Development", "name": "Human Erythropoietin (EPO) Alpha-Helix A Secretory Leader", "organism_table": "Homo sapiens (9606)", "accession": "NM_000799.4:182..235", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MGVHECPAWLWLLLSLLS", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.71, "optimized_cai": 1.0, "wildtype_gc3_pct": 61.1111, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 35.1852, "optimized_uridine_pct": 20.3704, "wildtype_cpg_count": 1, "optimized_cpg_count": 3, "gen1_placeholder_mfe_error_kcal": 14.4689, "native_wt_mfe_kcal": -8.4, "viennarna_wt_mfe_kcal": -8.4, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -19.7, "viennarna_opt_mfe_kcal": -19.7, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -20.5, "viennarna_opt_ensemble_kcal": -20.5, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -11.3, "tir5_window_mfe_kcal": -0.9, "wildtype_mfe_structure": "..(((...(((.(((.((((.((...)).).))))))...))))))........", "optimized_mfe_structure": "..(((.((((....)))))))((..((((..(((....))).)))).)).....", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.710 -> 1.000, ΔMFE = -11.30 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 9.715, "viennarna_runtime_ms": 71.9567 }, { "cohort_id": "DEV-04", "split": "Development", "name": "Aequorea victoria Enhanced GFP (eGFP) Fluorophore Beta-Can Core", "organism_table": "Homo sapiens (9606)", "accession": "U55762.1:280..333", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MVSKGEELFTGVVPILVE", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.6423, "optimized_cai": 1.0, "wildtype_gc3_pct": 33.3333, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 35.1852, "optimized_uridine_pct": 18.5185, "wildtype_cpg_count": 0, "optimized_cpg_count": 3, "gen1_placeholder_mfe_error_kcal": 15.0689, "native_wt_mfe_kcal": -7.4, "viennarna_wt_mfe_kcal": -7.4, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -19.1, "viennarna_opt_mfe_kcal": -19.1, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -20.08, "viennarna_opt_ensemble_kcal": -20.08, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -11.7, "tir5_window_mfe_kcal": -0.6, "wildtype_mfe_structure": "..........(((((..(((((..............)))))..)))))......", "optimized_mfe_structure": "..((((((((...((.....))))))))))(((.....)))..(((....))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.642 -> 1.000, ΔMFE = -11.70 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 7.1691, "viennarna_runtime_ms": 29.8746 }, { "cohort_id": "DEV-05", "split": "Development", "name": "Streptococcus pyogenes Cas9 RuvC-I Catalytic Motif (mRNA Editor Cargo)", "organism_table": "Homo sapiens (9606)", "accession": "NC_002737.2:854750..854803", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MDKKYSIGLDIGTNSVGW", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.7367, "optimized_cai": 1.0, "wildtype_gc3_pct": 22.2222, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 35.1852, "optimized_uridine_pct": 12.963, "wildtype_cpg_count": 1, "optimized_cpg_count": 3, "gen1_placeholder_mfe_error_kcal": 21.9689, "native_wt_mfe_kcal": -7.2, "viennarna_wt_mfe_kcal": -7.2, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -12.2, "viennarna_opt_mfe_kcal": -12.2, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -13.43, "viennarna_opt_ensemble_kcal": -13.43, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -5.0, "tir5_window_mfe_kcal": 0.0, "wildtype_mfe_structure": "............(((((.((.(((((((.........))))))).)).))))).", "optimized_mfe_structure": ".(((....((.((.((((....)).)).)).))....))).((((....)))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.737 -> 1.000, ΔMFE = -5.00 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 5.7865, "viennarna_runtime_ms": 28.8917 }, { "cohort_id": "DEV-06", "split": "Development", "name": "Zero-Degeneracy Met-Trp (AUG-UGG) Repeat Control (0-Beaker Gate)", "organism_table": "Synthetic Non-Degenerate Control", "accession": "SIM-NONDEGEN-DEV06", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MWMWMWMWMWMWMWMWMW", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 1.0, "optimized_cai": 1.0, "wildtype_gc3_pct": 100.0, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 33.3333, "optimized_uridine_pct": 33.3333, "wildtype_cpg_count": 0, "optimized_cpg_count": 0, "gen1_placeholder_mfe_error_kcal": 29.5689, "native_wt_mfe_kcal": -4.6, "viennarna_wt_mfe_kcal": -4.6, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -4.6, "viennarna_opt_mfe_kcal": -4.6, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -5.75, "viennarna_opt_ensemble_kcal": -5.75, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": 0.0, "tir5_window_mfe_kcal": 0.0, "wildtype_mfe_structure": "....(.((.((.((.((.((.((.((....)).)).)).)).)).)).)).)..", "optimized_mfe_structure": "....(.((.((.((.((.((.((.((....)).)).)).)).)).)).)).)..", "regime_classification": "0-Beaker Non-Degenerate / Pre-Saturated Control Rejected (ΔCAI = 0.0000, ΔMFE = 0.00 kcal/mol)", "concordant_with_viennarna": true, "native_runtime_ms": 6.0286, "viennarna_runtime_ms": 28.4841 }, { "cohort_id": "RES-01", "split": "Reserve", "name": "Respiratory Syncytial Virus (RSV) Prefusion F Antigenic Site Zero (mRNA-1345)", "organism_table": "Homo sapiens (9606)", "accession": "NC_001781.1:5840..5893", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MNINENIKNDLLSNNKLL", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.5819, "optimized_cai": 1.0, "wildtype_gc3_pct": 11.1111, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 37.037, "optimized_uridine_pct": 12.963, "wildtype_cpg_count": 0, "optimized_cpg_count": 2, "gen1_placeholder_mfe_error_kcal": 31.2689, "native_wt_mfe_kcal": -5.3, "viennarna_wt_mfe_kcal": -5.3, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -2.9, "viennarna_opt_mfe_kcal": -2.9, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -4.66, "viennarna_opt_ensemble_kcal": -4.66, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": 2.4, "tir5_window_mfe_kcal": 0.0, "wildtype_mfe_structure": "........................(((((((((((.......))))))))))).", "optimized_mfe_structure": "....................................((((.(.....).)))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.582 -> 1.000, ΔMFE = +2.40 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 5.2757, "viennarna_runtime_ms": 36.4267 }, { "cohort_id": "RES-02", "split": "Reserve", "name": "Rabies Lyssavirus Glycoprotein G Neutralizing Epitope (CV7202 mRNA)", "organism_table": "Homo sapiens (9606)", "accession": "NC_001542.1:3318..3371", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MVPQALFVPLIVFFSFVL", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.5668, "optimized_cai": 1.0, "wildtype_gc3_pct": 11.1111, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 61.1111, "optimized_uridine_pct": 31.4815, "wildtype_cpg_count": 0, "optimized_cpg_count": 3, "gen1_placeholder_mfe_error_kcal": 24.5689, "native_wt_mfe_kcal": -1.6, "viennarna_wt_mfe_kcal": -1.6, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -9.6, "viennarna_opt_mfe_kcal": -9.6, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -11.05, "viennarna_opt_ensemble_kcal": -11.05, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -8.0, "tir5_window_mfe_kcal": -5.3, "wildtype_mfe_structure": "..........(((..(.....)..)))...........................", "optimized_mfe_structure": "..((.(((...))))).....((((..((((.........))))....))))..", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.567 -> 1.000, ΔMFE = -8.00 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 5.5388, "viennarna_runtime_ms": 69.6188 }, { "cohort_id": "RES-03", "split": "Reserve", "name": "Human Coagulation Factor IX (F9) Gla-Domain Replacement mRNA", "organism_table": "Homo sapiens (9606)", "accession": "NM_000133.4:30..83", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MQRVNMIMAESPGLITIA", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.8194, "optimized_cai": 1.0, "wildtype_gc3_pct": 66.6667, "optimized_gc3_pct": 94.4444, "wildtype_uridine_pct": 20.3704, "optimized_uridine_pct": 14.8148, "wildtype_cpg_count": 2, "optimized_cpg_count": 3, "gen1_placeholder_mfe_error_kcal": 13.9689, "native_wt_mfe_kcal": -10.3, "viennarna_wt_mfe_kcal": -10.3, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -20.2, "viennarna_opt_mfe_kcal": -20.2, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -20.81, "viennarna_opt_ensemble_kcal": -20.81, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -9.9, "tir5_window_mfe_kcal": 0.0, "wildtype_mfe_structure": "....((((.((((...(((...(((........)))....)))))))...))))", "optimized_mfe_structure": ".........((((...((((((.(((((.......)))))))))))...)))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.819 -> 1.000, ΔMFE = -9.90 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 3.9271, "viennarna_runtime_ms": 27.5813 }, { "cohort_id": "RES-04", "split": "Reserve", "name": "Human CFTR NBD1 Walker-A ATP-Binding Loop (mRNA Ciliary Therapy)", "organism_table": "Homo sapiens (9606)", "accession": "NM_000492.4:1360..1413", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MFGVSYDEYRYRSVIKAC", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.7859, "optimized_cai": 1.0, "wildtype_gc3_pct": 38.8889, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 31.4815, "optimized_uridine_pct": 18.5185, "wildtype_cpg_count": 1, "optimized_cpg_count": 5, "gen1_placeholder_mfe_error_kcal": 21.9689, "native_wt_mfe_kcal": -7.6, "viennarna_wt_mfe_kcal": -7.6, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -12.2, "viennarna_opt_mfe_kcal": -12.2, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -13.47, "viennarna_opt_ensemble_kcal": -13.47, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -4.6, "tir5_window_mfe_kcal": -2.5, "wildtype_mfe_structure": "(((((((((((((((((((.....))))).....)))))))))....)))))..", "optimized_mfe_structure": "...((((.((((...)))).))))..(((((....((......))...))))).", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.786 -> 1.000, ΔMFE = -4.60 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 6.1186, "viennarna_runtime_ms": 30.5553 }, { "cohort_id": "RES-05", "split": "Reserve", "name": "Photinus pyralis Firefly Luciferase (Fluc) Adenylate-Forming Core", "organism_table": "Homo sapiens (9606)", "accession": "M15077.1:253..306", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MEDAKNIKKGPAPFYPLE", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 0.7167, "optimized_cai": 1.0, "wildtype_gc3_pct": 61.1111, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 12.963, "optimized_uridine_pct": 11.1111, "wildtype_cpg_count": 4, "optimized_cpg_count": 2, "gen1_placeholder_mfe_error_kcal": 23.2689, "native_wt_mfe_kcal": -10.7, "viennarna_wt_mfe_kcal": -10.7, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -10.9, "viennarna_opt_mfe_kcal": -10.9, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -12.02, "viennarna_opt_ensemble_kcal": -12.02, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": -0.2, "tir5_window_mfe_kcal": -1.6, "wildtype_mfe_structure": ".........(((...............)))((((((..........))))))..", "optimized_mfe_structure": ".(((......))).........(((((((.......)))))))...........", "regime_classification": "Synonymous Lattice Stabilized (CAI 0.717 -> 1.000, ΔMFE = -0.20 kcal/mol, 100% AA Identity)", "concordant_with_viennarna": true, "native_runtime_ms": 3.9987, "viennarna_runtime_ms": 27.1978 }, { "cohort_id": "RES-06", "split": "Reserve", "name": "Reserve Pre-Saturated Optimal Codon Control (0-Beaker Gate)", "organism_table": "Synthetic Pre-Optimized Control", "accession": "SIM-PRESAT-RES06", "num_codons": 18, "cds_length_nt": 54, "amino_acid_sequence": "MFYLVDEMNFYLVDEMNF", "aa_identity_preserved_pct": 100.0, "wildtype_cai": 1.0, "optimized_cai": 1.0, "wildtype_gc3_pct": 100.0, "optimized_gc3_pct": 100.0, "wildtype_uridine_pct": 27.7778, "optimized_uridine_pct": 27.7778, "wildtype_cpg_count": 2, "optimized_cpg_count": 2, "gen1_placeholder_mfe_error_kcal": 18.4689, "native_wt_mfe_kcal": -15.7, "viennarna_wt_mfe_kcal": -15.7, "abs_diff_wt_mfe_kcal": 0.0, "native_opt_mfe_kcal": -15.7, "viennarna_opt_mfe_kcal": -15.7, "abs_diff_opt_mfe_kcal": 0.0, "native_opt_ensemble_kcal": -17.39, "viennarna_opt_ensemble_kcal": -17.39, "abs_diff_opt_ensemble_kcal": 0.0, "delta_mfe_opt_minus_wt_kcal": 0.0, "tir5_window_mfe_kcal": -2.5, "wildtype_mfe_structure": "....((((((.((((((.(.......).)))))).)))))).............", "optimized_mfe_structure": "....((((((.((((((.(.......).)))))).)))))).............", "regime_classification": "0-Beaker Non-Degenerate / Pre-Saturated Control Rejected (ΔCAI = 0.0000, ΔMFE = 0.00 kcal/mol)", "concordant_with_viennarna": true, "native_runtime_ms": 4.8972, "viennarna_runtime_ms": 28.445 } ], "ascii_summary_table": "========================================================================================================================================================\n TOOL BENCHMARK #20: Native Synonymous Codon & RNA MFE Optimizer vs. Live ViennaRNA v2.7.2 (RNAfold -p --noPS)\n========================================================================================================================================================\nCohort Split WT CAI Opt CAI WT GC3% Opt GC3% WT U% Opt U% Nat Opt MFE Vienna MFE |ΔMFE| Nat Ens dG Vienna Ens AA Id% Match\n--------------------------------------------------------------------------------------------------------------------------------------------------------\nDEV-01 Development 0.7170 1.0000 33.33% 100.00% 37.0% 14.8% -13.30 -13.30 0.0000 -14.56 -14.56 100.0% YES (100%)\nDEV-02 Development 0.5630 1.0000 16.67% 100.00% 37.0% 18.5% -20.10 -20.10 0.0000 -21.54 -21.54 100.0% YES (100%)\nDEV-03 Development 0.7100 1.0000 61.11% 100.00% 35.2% 20.4% -19.70 -19.70 0.0000 -20.50 -20.50 100.0% YES (100%)\nDEV-04 Development 0.6423 1.0000 33.33% 100.00% 35.2% 18.5% -19.10 -19.10 0.0000 -20.08 -20.08 100.0% YES (100%)\nDEV-05 Development 0.7367 1.0000 22.22% 100.00% 35.2% 13.0% -12.20 -12.20 0.0000 -13.43 -13.43 100.0% YES (100%)\nDEV-06 Development 1.0000 1.0000 100.00% 100.00% 33.3% 33.3% -4.60 -4.60 0.0000 -5.75 -5.75 100.0% YES (100%)\nRES-01 Reserve 0.5819 1.0000 11.11% 100.00% 37.0% 13.0% -2.90 -2.90 0.0000 -4.66 -4.66 100.0% YES (100%)\nRES-02 Reserve 0.5668 1.0000 11.11% 100.00% 61.1% 31.5% -9.60 -9.60 0.0000 -11.05 -11.05 100.0% YES (100%)\nRES-03 Reserve 0.8194 1.0000 66.67% 94.44% 20.4% 14.8% -20.20 -20.20 0.0000 -20.81 -20.81 100.0% YES (100%)\nRES-04 Reserve 0.7859 1.0000 38.89% 100.00% 31.5% 18.5% -12.20 -12.20 0.0000 -13.47 -13.47 100.0% YES (100%)\nRES-05 Reserve 0.7167 1.0000 61.11% 100.00% 13.0% 11.1% -10.90 -10.90 0.0000 -12.02 -12.02 100.0% YES (100%)\nRES-06 Reserve 1.0000 1.0000 100.00% 100.00% 27.8% 27.8% -15.70 -15.70 0.0000 -17.39 -17.39 100.0% YES (100%)\n--------------------------------------------------------------------------------------------------------------------------------------------------------\nSUMMARY: Dev |ΔMFE|=0.0000, Res |ΔMFE|=0.0000 kcal/mol | MFE r=1.000000, Ens r=1.000000 | CAI 0.6840->1.0000 | Speedup=10.5x\n" }