{
  "study_id": "STUDY-COLEOPTERA-PCWDE-2026",
  "preprint_id": "PREPRINT-13",
  "version": "2.0.0",
  "title": "Permian–Cenozoic Coleoptera Phylogenomics (308.0 to 0.0 Ma): Resolving Archostemata–Myxophaga–Adephaga–Polyphaga Divergence, Strepsiptera Compositional Long-Branch Attraction, and Horizontal Gene Transfer of Microbial Plant Cell-Wall Degrading Enzymes (GH5, GH28, GH45) via RuNIE",
  "tier": 3,
  "tier_label": "Tier 3: Scientific",
  "beaker_prior": 5,
  "beaker_posterior": 5,
  "runie_replicates": 1000,
  "total_records": 101,
  "total_taxa": 83,
  "total_raw_bp": 1378439,
  "empirical_mitogenome_records": 83,
  "empirical_mitogenome_bp": 1354809,
  "nuclear_pcwde_records": 18,
  "nuclear_pcwde_bp": 23630,
  "full_length_alignments": {
    "mafft_v7526_mito_cols": 35624,
    "mafft_v7526_mito_cpu_sec": 896.99,
    "mafft_v7526_nuc_cols": 3321,
    "mafft_v7526_nuc_cpu_sec": 2.08,
    "mafft_v7526_total_evidence_cols": 38945,
    "mafft_v7526_total_evidence_cpu_sec": 899.07,
    "native_gotoh_msa_ry3_cpu_sec": null,
    "native_gotoh_msa_raw_cpu_sec": null
  },
  "codon_alignment_4gene": {
    "loci": [
      "COX1 (514 codons = 1,542 bp)",
      "COB (375 codons = 1,125 bp)",
      "COX2 (227 codons = 681 bp)",
      "COX3 (259 codons = 777 bp)"
    ],
    "total_codons": 1375,
    "total_bp": 4125,
    "total_evidence_bp": 5727
  },
  "rcfv_metrics": {
    "whole_mitogenome_4state_nt": 0.0624,
    "pos3_4gene_nt": 0.0312,
    "unrecoded_4gene_cds_nt": 0.0146,
    "pos12_4gene_nt": 0.0089,
    "ry3_recoded_cds_nt": 0.0074,
    "dayhoff6_aa": 0.0034
  },
  "blinded_dual_pass": {
    "mode": "Dual-Pass Blinded K2P Clade-Separation Permutation Audit (B = 500)",
    "total_pairs": 3403,
    "within_clade_pairs": 773,
    "between_clade_pairs": 2630,
    "mean_within_k2p": 0.2966,
    "mean_between_k2p": 0.3312,
    "z_blind": 3.81,
    "z_ctx": 3.81,
    "d_ctx": 0,
    "exact_refseq_genbank_duplicate_detected": "T007 (NC_011328.1) == T071 (EU877953.1) Tetraphalerus bruchi (15,689 bp, K2P = 0.0000)"
  },
  "pipeline_a_total_evidence_5727bp": {
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  "pipeline_b_mito83_4125bp": {
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      "taxon": "Paleosepharia bicavipennis",
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      "taxon": "Arctolamia fruhstorferi",
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      "taxon": "Gastrolina depressa",
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      "header": "NC_085605.1 Gastrolina depressa mitochondrion, complete genome",
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      "taxon": "Trichochrysea japana",
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      "header": "NC_082905.1 Trichochrysea japana mitochondrion, complete genome",
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      "taxon": "Agetocera filicornis",
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      "header": "NC_082137.1 Agetocera filicornis mitochondrion, complete genome",
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      "taxon": "Bruchus pisorum",
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      "taxon": "Batocera horsfieldi",
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      "header": "NC_080229.1 Batocera horsfieldi isolate tissue mitochondrion, complete genome",
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      "taxon": "Luperomorpha xanthodera",
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      "header": "NC_077572.1 Luperomorpha xanthodera mitochondrion, complete genome",
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      "taxon": "Rhynchophorus phoenicis",
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      "header": "NC_083152.1 Rhynchophorus phoenicis voucher VS9815 mitochondrion, complete genome",
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      "taxon": "Curculio bimaculatus",
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      "header": "NC_082136.1 Curculio bimaculatus mitochondrion, complete genome",
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      "taxon": "Hylurgus ligniperda",
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      "taxon": "Tomicus yunnanensis",
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      "taxon": "Corigetus marmoratus",
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      "header": "NC_073111.1 Corigetus marmoratus mitochondrion, complete genome",
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      "taxon": "Callirhopalus sedakowii",
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      "header": "NC_064330.1 Callirhopalus sedakowii mitochondrion, complete genome",
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      "taxon": "Dryocoetes hectographus",
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      "header": "XM_076416491.1 PREDICTED: Rhynchophorus ferrugineus polygalacturonase (LOC143204052), mRNA",
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      "header": "XM_076416156.1 PREDICTED: Rhynchophorus ferrugineus polygalacturonase (LOC143203845), mRNA",
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      "header": "XM_076416153.1 PREDICTED: Rhynchophorus ferrugineus polygalacturonase (LOC143203843), mRNA",
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      "header": "XM_076416151.1 PREDICTED: Rhynchophorus ferrugineus polygalacturonase (LOC143203840), mRNA",
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      "header": "XM_076407000.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase (LOC143198059), mRNA",
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      "header": "XM_076400312.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase F (LOC143193877), transcript variant X5, mRNA",
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      "header": "XM_076400311.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase F (LOC143193877), transcript variant X4, mRNA",
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      "header": "XM_076400310.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase F (LOC143193877), transcript variant X3, mRNA",
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    {
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      "header": "XM_076400309.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase F (LOC143193877), transcript variant X2, mRNA",
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    {
      "acc": "XM_076400308.1",
      "taxon": "Rhynchophorus ferrugineus",
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      "clade": "Phytophaga HGT Loci",
      "header": "XM_076400308.1 PREDICTED: Rhynchophorus ferrugineus endoglucanase F (LOC143193877), transcript variant X1, mRNA",
      "len": 2479,
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      "acc": "NC_011277.1",
      "taxon": "Ascaloptynx appendiculata",
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}